Part:BBa_K4780000:Design

This is a synthetic arsenic binding protein based designed using the protein MPNN neural network.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 84
  Illegal NgoMIV site found at 176
  Illegal AgeI site found at 284
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The key arsenic binding residues were held constant and the algorithm was able to alter the rest of the sequence.

Source

This is a protein MPNN variant of the ArsR gene from A. ferrooxidans. This is the server used to generate the sequence. https://huggingface.co/spaces/simonduerr/ProteinMPNN